Titlebook: Microinjection; Methods and Protocol Chengyu Liu,Yubin Du Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Gene

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Transgene Recombineering in Bacterial Artificial Chromosomes,ACs due to their size and complexity, thus a specialized field “recombineering” has developed to modify BAC clones through the use of homologous recombination in bacteria with short homology regions. Genetically engineered BACs can then be used in cell culture, mouse, or rat models to study cancer, neurology, and genetics.

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1064-3745 er half centered on a wide variety of other models.Contains This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impac

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Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method,petent to produce offspring. This can be exploited for the generation of genetically modified mice, through the transplantation of genetically modified GS cells. In this section, we describe a method of genome editing-mediated GS cell modification and transplantation.

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Generation of Rabbit Models by Gene Editing Nucleases, now possible to produce gene targeting (i.e., knockout, knockin) rabbits with high success rates. In this chapter, we describe a comprehensive, step-by-step protocol for rabbit genome editing based on gene editing nucleases with specific emphasis of CRISPR/Cas9.

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Book 2019ut also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-edit