Titlebook: Microinjection; Methods and Protocol Chengyu Liu,Yubin Du Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Gene

Genteel

心胸狹窄

Allege

托運(yùn)

xanthelasma

Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique,in pronuclear injection. Moreover, Cytochalasin B that is used to soften zygotes during piezo-driven microinjection has been suggested a role in improving the knockin efficiency, which provides an additional benefit to use this injection method.

吊胃口

Processes

蕁麻

The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Speathione to the fertilization medium. These modifications greatly enhanced the fertility of frozen-thawed C57BL/6 sperm, resulting in a stable fertilization rate >80% in IVF. Our results indicate that this robust protocol for sperm cryopreservation may improve the archiving and distributing system fo

Anticlimax

Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line,and single hepatocytes (both genome-edited and wild-type) can be isolated for analysis immediately following transient gene editing. Previously, we demonstrated successful isolation of genome-edited single hepatocytes, using mice expressing systemic Cas9 transgene (called “sCAT” mouse) and by tail-v

強(qiáng)壯