Titlebook: Membrane Biochemistry; A Laboratory Manual Ernesto Carafoli,Giorgio Semenza Book 1979 Springer-Verlag New York Inc. 1979 Calcium.Chloropla

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Nonelectrolyte Transport in Small Intestinal Membrane Vesicles. The Application of Filtration for Tsolic constituents and metabolites, the composition of the internal and the external compartment can be decided upon (within limits) by the experimenter according to the experimental requirements, (b) Brush border vesicles are a suitable starting material for isolating membrane components.

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Commonplace

Book 1979resents the experience accumulated during four advanced courses held at the Depart- ment of Biochemistry of the Swiss Federal Institute of Technology on behalf of Federation of European Biochemical Societies (FEBS) in the years 1975 through 1978. The idea of collecting the experiments into a laborat

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Preparation and Assay of Animal Mitochondria and Submitochondrial Vesicles,er membrane. Due to the low ionic conductance of a gradient of electrochemical potential is formed across the membrane and utilized by mitochondrial ATPase to synthesize ATP. The number of ATP molecules synthesized per pair of electrons which flow down the respiratory chain from substrate to oxygen

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Proton Translocation Catalyzed by Mitochondrial Cytochrome Oxidase,eing organized in so-called loops, in which system the terminal segment of the chain, viz. cytochrome c oxidase, would simply function as a transmembrane electron translocator. However, as shown by Wikstr?m and collaborators (Wikstr?m, 1977; Wikstr?m and Saari, 1977; Krab and Wikstr?m, 1978), cytoch

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Oxygen Evolution and Uptake as a Measure of the Light-Induced Electron Transport in Spinach Chloropy an electron transport chain. This scheme and a possible arrangement in the thylakoids proposed by Trebst (1974) is summarized in Figure 1. In Figure 2 some possible electron donors, acceptors, and inhibitors are introduced which will be used in the experiments of this course. With these artificial

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Book 1979 took shape, to accommodate to experience and various suggestions. In their present form, all of the experiments described have been repeatedly practiced to optimize their execution. Efforts have been made to combine in the manual classical experiments, and techniques which require relatively unsoph