Titlebook: Membrane Biochemistry; A Laboratory Manual Ernesto Carafoli,Giorgio Semenza Book 1979 Springer-Verlag New York Inc. 1979 Calcium.Chloropla

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Markus Kessler,Gerhard Toggenburgerh theoretical reflections and good-practice descriptions in Pre-university engineering education has become the topic of increasing interest in technology education circles. It can provide content for the E in STEM (Science, Technology, Engineering and Mathematics) education, which is in the interes

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Nonelectrolyte Transport in Small Intestinal Membrane Vesicles. The Application of Filtration for Tn important step forward in two respects: (a) These vesicles allow to dissect clearly phenomena occurring in the brush border membrane from others occurring in the cell interior or in other membranes, as, for example, in the basal-lateral membrane. In these vesicles, which are virtually free of cyto

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Transport of Sugars in Bacteria,n carbon source for their nutrition and the selective advantage of metabolizing fast any of these for fast proliferation promoted the development along the evolution of different adaptive uptake and catabolic equipments. The aim of the experiments presented here is to make acquaintance with two typi

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Calcium Transport in Resealed Erythrocytes and the Use of a Calcium-Sensitive Electrode,ient is maintained by the low permeability of the membrane toward Ca. and by an ATPase usually called (Ca. + Mg.)-ATPase or Ca,Mg-ATPase, which pumps Ca. in the outward direction. The pump capacity exceeds the passive leak flux by a factor of at least 100. An activator protein of the Ca,Mg-ATPase ha

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Preparation and Assay of Animal Mitochondria and Submitochondrial Vesicles,lites. The energy-rich ATP molecule is then used as the energy donor for the different cellular functions. Mitochondria contain several membrane-bound redox components (flavoproteins, iron-sulfur proteins, ubiquinone-10, and cytochromes) which catalyze the reduction of molecular oxygen by the reduce

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Measurement of Cytochrome Kinetics in Rat Liver Mitochondria by Stopped Flow and Dual-Wavelength Sption to an anaerobic mitochondrial suspension. The stopped-flow analysis of mitochondrial electron carriers allows also to distinguish which of the carriers are “kinetically competent,” i.e., belong to the main oxidation-reduction pathway of the respiratory chain, from those which are located in sid

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