Titlebook: Meaning in Mathematics Education; Jeremy Kilpatrick,Celia Hoyles,Paola Valero Book 2005 Springer-Verlag US 2005 algebra.communication.educ

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978-1-4419-3682-0Springer-Verlag US 2005

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e then discuss methods for analyzing these models with a focus on isolating cell-type-specific changes and mechanism investigation. Specifically, we describe lentiviral transduction and flow cytometry as established and robust methods to identify and separate each cell type for downstream analysis.

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Jeremy Kilpatrick,Celia Hoyles,Ole Skovsmose,Paola Valerops on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Ovarian Cancer: Methods and Protocols. serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find newtherapeutic approaches..978-1-0716-1958-2978-1-0716-1956-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Maria G. Bartolini Bussieeping the objective lens and thus the optically trapped microspheres in a fixed position. In this chapter, we describe design guidelines for a fluorescence imaging module on a Q-trap system that overcomes these challenges and provide a step-by-step description for construction and alignment of such

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Rolf Biehler generating ssDNA of up to 1000 nucleotides as well as more complex structures, such as a 120-base-pair DNA hairpin positioned next to a 33-nucleotide ssDNA segment. The utility of the hairpin substrate was demonstrated by measuring the motion of . RecQ, a 3′-to-5′ DNA helicase.

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Ole Skovsmoseesolve helicase heterogeneity at the single-molecule level. In this chapter, we describe a single-molecule method that combines optical tweezers with confocal fluorescence microscopy to study helicase-catalyzed DNA unwinding. Using Bloom syndrome protein (BLM), a multifunctional helicase that mainta

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Jeremy Kilpatrick,Celia Hoyles,Ole Skovsmose,Paola Valeroe multicolor fluorescence capability allows the direct observation of multiple molecules or multiple degrees of freedom which allows, for example, the observation of multiple proteins simultaneously within a complex. The instrument incorporates three fluorescence excitation lasers, all sourced from