Titlebook: Mass Spectrometry-Based Proteomics; Kris Gevaert Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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tion erl?utert. Zun?chst wird auf einen günstigen Entwurf von Zwischenfrequenzfiltern in Hinblick auf minimale Verzerrungen eingegangen. Dabei werden digitale nichtrekursive Filter zugrunde gelegt, die streng linearphasig entworfen werden k?nnen und somit keinerlei Phasenverzerrungen einbringen. Im

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Studying Cellular Dynamics Using Proximity-Dependent Biotinylation: Somatic Cell Reprogramming,nducible lentiviral toolkit for BioID, we define here protocols for time-resolved PDB in primary cells. Here, we report the optimization and application of lentivirally delivered miniTurbo constructs to a mouse fibroblast model of somatic cell reprogramming, allowing the study of this dynamic proces

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Virotrap: Trapping Protein Complexes in Virus-Like Particles,and biotin-dependent identification (BioID), Virotrap was shown to complement data obtained with the existing MS-based toolkit. The proven complementarity of these MS-based strategies underlines the importance of using different techniques to enable comprehensive mapping of protein-protein interacti

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Systems-Wide Site-Specific Analysis of Glycoproteins,description of the methods that include procedures for (i) proteolytic digestion using specific proteases, (ii) optional glycopeptide enrichment using hydrophilic interaction liquid chromatography, (iii) nano-LC-MS/MS analysis of glycopeptides, and (iv) data analysis for identification of glycopepti

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Book 2023sts of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Mass Spectrometry-Based Proteomics. serves as an ideal guide to its subject for both novices in the field o

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