Titlebook: Mass Spectrometry-Based Proteomics; Kris Gevaert Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spr

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Studying Cellular Dynamics Using Proximity-Dependent Biotinylation: Somatic Cell Reprogramming, the mechanistic underpinning of morphological and fate changes associated with these processes. The advent of proximity-dependent biotinylation (PDB) methods has overcome?some of?the limitations of biochemical purification methods, enabling proteomic characterization of most subcellular compartment

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Sensitive and High-Throughput Exploration of Protein N-Termini by TMT-TAILS N-Terminomics,the characterization of physiological or proteolytically processed protein N-termini, as well as other N-terminal posttranslational modifications (PTMs). TAILS is a well-established, high-throughput, negative enrichment workflow that enables system-wide exploration of N-terminomes independent of sam

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The Global Acetylation Profiling Pipeline for Quick Assessment of Protein N-Acetyltransferase Speciable sensitivity and robustness of the GAP pipeline relies on the very low frequency of known N-terminal acetylated proteins in ., including their degree of N-terminal acetylation. Using the SILProNAQ mass spectrometry strategy on bacterial protein extracts, GAP permits easy acquisition of both qual

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Proteomics-Based Analysis and Diagnosis of Formalin-Fixed Paraffin-Embedded Amyloidosis Samples,f amyloidosis often relies on immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) patient samples; however, dependency on antibodies for protein staining is one of the major pitfalls of this approach, especially for the detection of rare amyloidosis types. In recent years, mass spectrome