Titlebook: Mass Spectrometry in Biology & Medicine; A. L. Burlingame,Steven A. Carr,Michael A. Baldwin Book 2000 Springer Science+Business Media New

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Isotopic Amplification, H/D Exchange, and Other Mass Spectrometric Strategies for Characterization mapped by mass spectrometry. First, isotopic amplification of proteins, achieved by expression from growth media doubly-depleted in carbon-13 and nitrogen-15, narrows, amplifies, and shifts the isotopic mass distribution, to identify definitively the ?monoisotopic“ species (i. e., the only species w

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Studying Noncovalent Small Molecule Interactions with Protein and RNA Targets by Mass Spectrometry,tical research. To develop new therapies at an increasing pace, more new drugs need to be synthesized or discovered, more novel macromolecular targets need to be identified, and compounds need to be characterized, screened and evaluated at a faster rate. Advances and application of combinatorial che

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Identification of Protein-Protein Interfaces by Amide Proton Exchange Coupled to MALDI-TOF Mass Spees. The method has broad application to the study of protein conformation and folding and to the study of protein-ligand interactions and requires no modifications of the instrument. Amide protons were allowed to exchange with deuterons in buffered D.O, pD 7.25 at room temperature. Exchanged deutero

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Electron Capture Dissociation Produces Many More Protein Backbone Cleavages Than Collisional and , avage products [R-C(OH)=NH + (n-m)H]. (.) + [·R’ + mH] . (.·), whereas conventional activation methods mainly cleave the amide bond R-C—/—NH-R’ to yield . and . ions. In the postulated cleavage mechanism, electron capture to form (M + nH).reduced molecular ions is followed by the transfer of the lab

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Protein Micro-Characterization by Mass Spectrometry: Sample Handling and Data Flow,s patterns of proteolytic fragments, or dissociation products thereof, to query databases [1–6]. Two issues, however, need to be addressed at the present time. Are we ready for high throughput analyses in a reliable, efficient and timely manner, and to apply these tools towards rationally selected,

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Towards an Integrated Analytical Technology for the Generation of Multidimensional Protein Expressit expressed by a tissue or differentiated cell [1]. In the most commonly used approach to proteome analysis the proteins extracted from the cell or tissue to be analyzed are separated by high resolution two dimensional gel electrophoresis (2DE), detected in the gel by staining or, if radiolabeled, b

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Sequencing the Primordial Soup,ar protein differences between whole cells (diseased vs. healthy) or between other complex biological fluids are important to understanding protein function. Once the structure and interactions of all proteins are characterized — in physiological and pathological situations — therapeutic strategies

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Coaxial Nanospray Coupled with a Hybrid Quadrupole/Time-of-Flight Tandem Mass Spectrometer for Prot pharmaceutical companies discover and develop drugs. These projects have two principal goals — the identification of the best disease genes/targets on which to initiate drug discovery programs, and the identification of patients who will respond favorably to drugs based on their genotype. To accomp

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