Titlebook: Mass Cytometry; Methods and Protocol Helen M. McGuire,Thomas M. Ashhurst Book 2019 Springer Science+Business Media, LLC, part of Springer N

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Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometryse, while retaining the possibility to deliver an in-depth characterization of antigen-specific CD8. T cells and associated phenotypes. Here we describe the method for a MHC class I tetramer multiplexing approach together with intracellular antibody staining for a parallel phenotypic cell characterization using mass cytometry in human specimens.

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Book 2019y reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Mass Cytometry: Methods and Protocols. aims to ensure successful results in the further study of this vital field..

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1064-3745 ation advice from the experts.This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly succ

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Book 2019 design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readil

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Diana Shinko,Thomas M. Ashhurst,Helen M. McGuire,Kellie A. Charles

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Automated Cell Processing for Mass Cytometry Experimentsy automated using a liquid handling robotic system and we present measures taken to optimize all steps of the protocol. These advances are applicable to both manual and automated protocols and provide a six-fold higher cell yield as compared to a standard protocol. With this increased yield and impr

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Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometryic system in the bone marrow (BM) is a critical component of both immunity and disease. Because of the complexity of the hematopoietic system in steady state and disease, high-dimensional cytometry technologies are well suited to the exploration of these complex systems. Here we describe a protocol

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Yannick Simoni,Michael Fehlings,Evan W. Newell the nuclei 19F and 15N, subvolume III/35C contains the nucleus 1H, subvolume III/35D contains the nucleus 13C, subvolume III/35E contains the nucleus 17O, and subvolume III/35G contains the nucleus 77Se. More nuclei will be presented later..978-3-540-74189-3Series ISSN 1615-1844 Series E-ISSN 1616-9522

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