Titlebook: Mass Cytometry; Methods and Protocol Helen M. McGuire,Thomas M. Ashhurst Book 2019 Springer Science+Business Media, LLC, part of Springer N

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書目名稱Mass Cytometry影響因子(影響力)




書目名稱Mass Cytometry影響因子(影響力)學(xué)科排名




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書目名稱Mass Cytometry網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Mass Cytometry被引頻次




書目名稱Mass Cytometry被引頻次學(xué)科排名




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書目名稱Mass Cytometry年度引用學(xué)科排名




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書目名稱Mass Cytometry讀者反饋學(xué)科排名

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1064-3745 eagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Mass Cytometry: Methods and Protocols. aims to ensure successful results in the further study of this vital field..978-1-4939-9454-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for ctrophotometry and frequently exceeds 60%. Each custom-conjugated antibody is validated using positive and negative cellular control populations and is titrated for optimal staining at concentrations ranging from 0.1 to 10?μg/mL. The preparation of metal-labeled antibodies can be completed in 4.5?h, and titration requires an additional 3–5?h.

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Mass Cytometric Cell Cycle Analysisration of 5-Iodo-2′-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), Cyclin B1, and phosphorylated Histone H3 (pHH3). These measurements can be integrated into a gating strategy that enables clear separation of all five phases of the cell cycle.

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TOXIN

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Acquisition, Processing, and Quality Control of Mass Cytometry Data cell measurements requires specific considerations in acquiring and processing data. This chapter provides an overview of how to optimally acquire mass cytometry data and how to process this data for subsequent analysis and characterization of cell populations.