Titlebook: Malaria Immunology; Targeting the Surfac Anja Tatiana Ramstedt Jensen,Lars Hviid Book 2022 The Editor(s) (if applicable) and The Author(s),

不要不誠實

Resetting , Gene Transcription in e PfEMP1 adhesive proteins, encoded by the . gene family. To avoid premature exposure of its antigenic repertoire, the parasite transcribes its . genes in a mutually exclusive manner, and switch expression at a very slow rate. This process is epigenetically regulated and it relies on “epigenetic mem

–DOX

META

978-1-0716-2191-2The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

顛簸下上

厭倦嗎你

anachronistic

Glucose

Immunomagnetic Selection of ,–Infected Erythrocytes Expressing Particular PfEMP1 Variants family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

Nmda-Receptor

absolve

CRISPR/Cas9 Editing of the , Genometions ranges from single point mutations to large multigene deletions, however many of the technical considerations for designing CRISPR-based experiments are common to any editing approach. This review will discuss protocols for vector construction and donor design for genome editing ., including pitfalls, variables, and validation methods.

表否定

Anja Tatiana Ramstedt Jensen,Lars HviidIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts