Titlebook: Malaria Immunology; Targeting the Surfac Anja Tatiana Ramstedt Jensen,Lars Hviid Book 2022 The Editor(s) (if applicable) and The Author(s),

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Immunomagnetic Selection of ,–Infected Erythrocytes Expressing Particular PfEMP1 Variants family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used fo

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Single-Cell Sorting of ,–Infected Erythrocytes Expressing Particular PfEMP1 Variantsclonal parasite population derived from a single parasite. This protocol describes an efficient method for cloning by using fluorescence-activated cell sorting (FACS). For this, an antibody for a particular IEs surface protein it is added to the cell mixture to separate positive and negative IEs for

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Selecting , Infected Erythrocytes for Adhesion to Cell Lines to evade host immunity. This enables IEs to sequester in the microvasculature of different organs and tissues of the host, contributing to different outcomes of disease. The in vitro study of cytoadhesion involves the use of IEs and human endothelial cells or other cell lines that express host cell

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Synchronisation of , and , In Vitro Cultures Using a Highly Specific Protein Kinase Inhibitoraria parasite life cycle. Here we describe a procedure using ML10, a highly specific inhibitor of the parasite cyclic GMP-dependent protein kinase (PKG), to attain high synchronicity of . and . asexual blood-stage cultures and to obtain high levels?of arrested mature schizonts as well as viable rele

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