Titlebook: Lymphoma; Methods and Protocol Ralf Küppers Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2019 Lase

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Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ-)PCR,lin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in

Heart-Attack

Stereotyped B Cell Receptor Immunoglobulins in B Cell Lymphomas, has led to the identification of significant repertoire restrictions, culminating in the discovery of subsets of patients expressing highly similar, stereotyped BcR IG. This finding strongly supports selection by common epitopes or classes of structurally similar epitopes in the ontogeny of these t

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Generation of Whole Genome Bisulfite Sequencing Libraries from Very Low DNA Input, DNA methylation changes are most effective in promoters and enhancers, the former frequently being CpG-rich and the latter, in contrast, CpG-poor. Many genome-wide methods for DNA methylation analysis interrogate predominantly CpG-rich regions and, hence, spare enhancers and other potentially impor

Conducive

FISH and FICTION in Lymphoma Research,uclei. It is widely used in routine diagnostics to identify cancer-specific aberrations including lymphoma-associated translocations or gene copy number changes in single tumor cells. By combining FISH with immunophenotyping—a technique called fluorescence immunophenotyping and interphase cytogeneti

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NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma,iRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in

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RNA Sequencing in B-Cell Lymphomas,NA (cDNA) to generate several millions of short sequence reads that are aligned to a reference genome allowing the comprehensive characterization of the transcripts in a cell. RNA-Seq has a wide variety of applications which lead to a pervasive adoption of this method well beyond the genomics commun

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Studying Cancer Heterogeneity by Single-Cell RNA Sequencing,e. Genetic and epigenetic changes in combination with external cues and selective forces are the driving factors behind tumor heterogeneity. Understanding this variability within and across patients may partly explain the unpredictable outcomes of cancer treatments. Measuring the variation of gene e

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Evaluation of Whole Genome Sequencing Data, analysis of WGS data, including alignment, identification of different classes of genomic variants, the identification of driver mutations, and the identification of mutational signatures. We further outline design considerations for WGS studies and provide a variety of quality control measures to