Titlebook: Lymphoma; Methods and Protocol Ralf Küppers Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature 2019 Lase

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Protocols for CRISPR-Cas9 Screening in Lymphoma Cell Lines,the CRISPR-Cas9 system for genome engineering has revolutionized functional genomic screening. Here, we present protocols used to introduce Cas9 into human lymphoma cell lines, produce high-titer lentivirus of a genome-wide sgRNA library, transduce and culture cells during the screen, isolate genomi

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Lymphoma and Leukemia Cell Vulnerabilities and Resistance Identified by Compound Library Screens,ion of biomarkers associated with response to drugs would greatly improve the efficacy of drug treatment. Platforms that can comprehensively map drug response in high-throughput ex vivo provide a unique tool to identify associated biomarkers and provide hypotheses for mechanisms underlying variable

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Proximity Ligation Assay, technique used to examine protein-protein interactions in situ. PLA offers many advantages over traditional biochemical approaches to signaling in that it can detect both transient interactions, which are hard to capture otherwise, as well as protein interactions within large supramolecular signali

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Ralf KüppersIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/589223.jpg

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Springer Science+Business Media, LLC, part of Springer Nature 2019

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Evaluation of Whole Genome Sequencing Data, analysis of WGS data, including alignment, identification of different classes of genomic variants, the identification of driver mutations, and the identification of mutational signatures. We further outline design considerations for WGS studies and provide a variety of quality control measures to detect common quality problems in the data.