Titlebook: Leptospira spp.; Methods and Protocol Nobuo Koizumi,Mathieu Picardeau Book 2020 Springer Science+Business Media, LLC, part of Springer Natu

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In Vivo Imaging of Bioluminescent Leptospires,This useful and accessible technology facilitates the study of the kinetics of blood and tissue dissemination of live leptospires, and the pharmacological impact of treatments and host directed therapeutics.

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Nobuo Koizumi,Mathieu PicardeauIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/l/image/585006.jpg

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Isolation and Culture of , from Clinical and Environmental Samples, leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of filtration, agar plating, and selective agents, leptospires can be isolated in pure cultures even from complex contaminated sources in standard EMJH culture medium.

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Isolation, Purification, and Characterization of Leptophages,he genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.

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