Titlebook: Kinesin Protocols; Isabelle Vernos Book 2001 Humana Press 2001

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Green Fluorescent Protein as a Tag for Molecular Motor Proteins,r in living cells when fused to another protein (.), GFP has come into wide use. GFP offers advantages over other reporters in that it fluoresces in live cells and its fluorescence does not require the addition of any substrates, cofactors, or accessory proteins. This means that GFP-tagged proteins

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The Use of Dominant Negative Mutants to Study the Function of Mitotic Motors in the In Vitro Spindlghly dynamic bipolar structure assembles and disassembles periodically and its main structural components are microtubules (MTs) and chromosomes. Microtubule-dependent motors in particular members of the kinesin superfamily play an important role in directing the self-organization of microtubules in

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Assaying Spatial Organization of Microtubules by Kinesin Motors,state, which makes them able to simultaneously bind two microtubules. Here, we describe an in vitro assay for the direct observation of the organization of microtubules by these motors. With this assay, patterns of microtubules are formed that reflect the morphogenetic abilities of the motors. The a

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Crystallization of Kinesin,ventional kinesin consists of two identical heavy chains carrying the motor activity, and two light chains. Based on its amino acid sequence, the heavy chain can be divided into three separate domains: (.) an N-terminal head, or motor domain, (.) an elongated a-helical stretch forming a coiled coil