Titlebook: Kinesin Protocols; Isabelle Vernos Book 2001 Humana Press 2001

祖先

Crystallization of Kinesin,y chain can be divided into three separate domains: (.) an N-terminal head, or motor domain, (.) an elongated a-helical stretch forming a coiled coil with a second kinesin heavy-chain polypeptide, (.) a C-terminal globular domain responsible for cargo binding and regulation of the motor’s activity (for reviews, ...–.).

旁觀者

1064-3745 end-directed cytoplasmic dynein, had been identified. At the time, these two motors seemed almost sufficient to explain directional motility events on polar microtubule tracks in the cell. No- theless, shortly after, the tip of the iceberg began to emerge with the identi- cation of proteins containi

cochlea

Preparation of Recombinant Kinesin Superfamily Proteins Using the Baculovirus System,e ATP-binding and the microtubule-binding domains of kinesin heavy chain, were cloned and characterized from murine brain libraries (.–.). They form kinesin superfamily proteins (KIFs). Now, 10 members (KIF1A, KIF1B, KIF2, KIF3A, KIF3B, KIF4, KIF5A, KIF5B, KIF5C, KIFC1, KIFC2, KIFC3) have been identified and characterized (.–.).

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救護車

Expression Cloning with Pan Kinesin Antibodies, peptide antibodies generated against two of these short, conserved sequences for the expression cloning of members of the kinesin-related protein family. We will briefly discuss the production of the pan-specific, kinesin peptide antisera and the four conserved regions within the motor domain of th

OPINE

Green Fluorescent Protein as a Tag for Molecular Motor Proteins, methods of fluorescent labeling of proteins. Moreover, GFP fluorescence is long-lasting, and its sensitivity to pH and temperature has enabled workers to use GFP fusion proteins to measure pH in specific cellular compartments (.) and to follow protein localization in temperature-shift experiments (