Titlebook: Innate DNA and RNA Recognition; Methods and Protocol Hans-Joachim Anders,Adriana Migliorini Book 2014 Springer Science+Business Media New Y

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1064-3745 ation advice from the experts.Innate DNA and RNA Recognition: Method and Protocols. presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions t

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Detection of RNA Modifications by HPLC Analysis and Competitive ELISAge can lead to random modifications in rRNA and messenger RNA (mRNA) that strongly impair functionality. RNA modifications have also been implicated in avoiding self-RNA recognition by the immune system or immune evasion by pathogens. Here, we describe the detection of RNA modifications by HPLC analysis and competitive ELISA.

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Analysis of Nucleic Acid-Induced Nonimmune Cell Death In Vitroo cell death. Here, we describe reliable and simple methods to quantify cell death caused by nucleic acid recognition. Additionally, we report two different methods to discriminate between two cell death modalities: apoptosis and necrosis.

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Isolation of Intratumoral Leukocytes of TLR-Stimulated Tumor-Bearing Miceysis (e.g., by flow cytometry) or cultured for functional in vitro studies. Here, we describe the isolation of intratumoral leukocytes by density gradient centrifugation. This technique can be used to isolate leukocytes from freshly dissected murine tumors.

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Viral Nucleic Acid Recognition in Human Nonimmune Cells: In Vitro Systemsemplify two viral infection models using cultured nonimmune cells and hepatitis C virus (HCV) or polyomavirus BK (BKV). In addition, we report the importance of siRNA technique in gene regulation in order to identify specific markers involved in innate antiviral responses in these cells.