Titlebook: Immunochemical Protocols; John D. Pound Book 19982nd edition Humana Press 1998

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cartilage

organic-matrix

DALLY

ENNUI

Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins,ncreasingly for expression of glycoproteins, because the recombinant material is glycosylated. It should be remembered, however, that glycosylation may be species-specific and if one of the functions of the protein depends on carbohydrate, then the function of a recombinant glycoprotein may be alter

overhaul

Spangle

Synthesis of Peptides for Use as Immunogens, antibody is already well defined (.,.) In this way, antibodies can be raised against novel gene products that are specifically directed against sites of interest, for example, unique regions, highly conserved regions, active sites, or extracellular or intracellular domains. Moreover, the ready avai

PHAG

IgG Purification,e supernatant or the isolation of a particular antigen-binding fraction of Ig from such fluids. The former can be achieved by biochemical procedures, whereas the latter usually requires some type of affinity purification.

放棄

Epitope Mapping,cted, and may depend also on the mapping method used. Epitope mapping is usually done with MAbs, though it can be done with polyclonal antisera in a rather less rigorous way, bearing in mind that antisera behave as a mixture of MAbs of variable complexity. Some authors have extended the epitope conc

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Coupling of Antibodies with Biotin,y of mannose and glucosamine (.), avidin can interact nonspecifically with negative charges on cell surfaces and nucleic acids, or with membrane sugar receptors. At times, this causes background problems in histochemical and cytochemical applications. Streptavidin, a near-neutral, biotin-binding pro