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Titlebook: Immunochemical Protocols; John D. Pound Book 19982nd edition Humana Press 1998

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書目名稱Immunochemical Protocols
編輯John D. Pound
視頻videohttp://file.papertrans.cn/463/462116/462116.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Immunochemical Protocols;  John D. Pound Book 19982nd edition Humana Press 1998
描述This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. ..The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.
出版日期Book 19982nd edition
版次2
doihttps://doi.org/10.1007/978-1-59259-257-9
isbn_ebook978-1-59259-257-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1998
The information of publication is updating

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Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase,?hler and Milstem (.) These workers have shown that lymphocytes can be immortalized and subsequently cultured after so1atic fusion with genetically selected myeloma cells. Usually, once fusion between spleen cells and myeloma cells has been performed, cells are suspended in a large volume of selecti
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Synthesis of Peptides for Use as Immunogens, cells or tissues. With the advent of recombinant DNA technology, it is now often possible to deduce the primary amino acid sequence of a polypeptide without its purification. Two strategies then exist to raise appropriate antibodies. The gene can be expressed in a heterologous species, usually bact
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IgG Purification,e supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin (Ig) is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity c
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