Titlebook: Ice Binding Proteins; Methods and Protocol Ran Drori,Corey Stevens Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusi

SSRIS

Measurement of Ice Nucleation Activity of Biological Samplesxperimental procedures vary with sample type. Here we describe a generalized primary purification and analysis process to measure ice nucleation activity of biological samples using an advanced freezing droplet assay.

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FOIL

Quantification of the Ice Nucleation Activity of Ice-Binding Proteins Using a Microliter Droplet Freopulation of droplets and observing their freezing temperatures. Here, a method for measuring the freezing temperatures of microliter-scale droplets is described and its application to quantifying a substances’ ice nucleation activity discussed.

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Measurement of Ice-Binding Protein Activity in Highly Alkaline Environmentst proteins, are sensitive to changes in their physical environment, which can alter their physical and chemical properties and ice-binding activities. Here we describe how to investigate IBP integrity for applications in alkaline environments and discuss incorporating IBPs into portland cement paste

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Measurement of Ice-Binding Protein Inhibition of Non-ice Crystal Growthtifreeze proteins (AFPs) are gaining attention in the natural gas research field due to their performance in crystal growth inhibition, excellent biodegradation, and low toxicity. Studies of AFPs may provide clues for developing future commercial KHIs used offshore. This chapter presents a simple me