Titlebook: Ice Binding Proteins; Methods and Protocol Ran Drori,Corey Stevens Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusi

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Multiscale Molecular Dynamics Simulations of Ice-Binding Proteinsmolecular dynamics simulation methodologies to investigate the mechanism of action of ice-binding proteins. Specifically, we provide detailed instructions to set up MD simulations to study the binding and interaction of ice-binding proteins using atomistic and coarse-grained simulations.

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https://doi.org/10.1007/978-1-0716-3503-2protein purification; Ice nucleation activity; dynamics simulations; polymer mimics; cryopreservation

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978-1-0716-3505-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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Measurement of Ice Nucleation Activity of Biological Samplesxperimental procedures vary with sample type. Here we describe a generalized primary purification and analysis process to measure ice nucleation activity of biological samples using an advanced freezing droplet assay.

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Ran Drori,Corey StevensIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/i/image/460701.jpg