Titlebook: High-Resolution Imaging of Cellular Proteins; Methods and Protocol Steven D. Schwartzbach,Omar Skalli,Thomas Schikors Book 2016 Springer Sc

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Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH-Sensitive Fluorescent Proteinsc vesicle endocytosis in response to extracellular stimulation in dissociated neuronal cultures of hippocampal neurons obtained from rats—also applicable to mice—using pHluorin-tagged vesicular glutamate transporter-1 as a reporter.

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Pre-embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cellsg embedded in an epoxy resin for ultrathin sectioning. The protocol also includes strategies for optimizing the balance between ultrastructure and antigen preservation, steps to minimize nonspecific antibody binding, and steps to optimize antibody penetration.

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1064-3745 ation advice from the experts.Includes supplementary materia.This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron

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Using FRAP or FRAPA to Visualize the Movement of Fluorescently Labeled Proteins or Cellular Organellactive transport. These two techniques have been extensively utilized to probe the cell biology of neurons. A practical outline of FRAP and FRAPA in cultured neurons is presented, including the preparation of the neurons and their infection with adeno-associated viral vectors. Considerations in planning such experiments are provided.

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Book 2016s used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluoresc