Titlebook: High-Resolution Imaging of Cellular Proteins; Methods and Protocol Steven D. Schwartzbach,Omar Skalli,Thomas Schikors Book 2016 Springer Sc

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Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab′)2, and Streptavells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.

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Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imagingn vivo 2P imaging presents significant challenges in sample preparation, which are exacerbated in non-murine species. Here, we describe procedures for the effective virally mediated labeling of neurons and for the implantation of cranial windows for imaging. The procedures described here are applica

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Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissuestructural preservation, as seen in the smooth plasma membrane, but also in the speed in arresting cell activity. Immunoelectron microscopy reveals the subcellular localization of molecules within cells. Immunolabeling in combination with cryofixation and freeze-substitution techniques provides more