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Titlebook: High Throughput Screening; Methods and Protocol William P. Janzen,Paul Bernasconi Book 2009Latest edition Humana Press 2009 Cellular and bi

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書目名稱High Throughput Screening
副標題Methods and Protocol
編輯William P. Janzen,Paul Bernasconi
視頻videohttp://file.papertrans.cn/427/426535/426535.mp4
概述Presents a comprehensive overview of screening techniques to create a How-to book for HTS.Contains detailed protocols for common HTS methods.Details protocols and techniques for cellular and biochemic
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: High Throughput Screening; Methods and Protocol William P. Janzen,Paul Bernasconi Book 2009Latest edition Humana Press 2009 Cellular and bi
描述.In the years since the release of the popular first edition, the field of High Throughput Screening (HTS) has evolved considerably, from a small niche area of study to a major, essential scientific technique. In .High Throughput Screening: Methods and Protocols, Second Edition., experts in the field fully update the previous edition to best serve as an introduction to HTS for scientists who are just entering the field, and as a provider of key, significant detail so as to be useful to scientists in established HTS operations. Featuring new screening technologies as well as many well established methods, chapters offer comprehensive treatment of the activities directly related to HTS, such as compound library management, data handling, and robotics. As a volume in the highly successful .Methods in Molecular Biology.? series, this work provides the kind of meticulous description and implementation advice that is crucial for getting optimal results...Authoritative and intrinsically practical, .High Throughput Screening: Methods and Protocols, Second Edition. promises to share the highest knowledge and enthusiasm with all those who use this versatile technology in their own labs..
出版日期Book 2009Latest edition
關鍵詞Cellular and biochemical assays; Chemical libraries; Compound library management; Data handling; HTS; Rob
版次2
doihttps://doi.org/10.1007/978-1-60327-258-2
isbn_softcover978-1-61779-491-9
isbn_ebook978-1-60327-258-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2009
The information of publication is updating

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Application of Fluorescence Polarization in HTS Assays,r interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.
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Design and Implementation of High-Throughput Screening Assays,ists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful analysis of many variables, starting with the choice of assay target and ending with the discovery of lead compounds. At every step in this process, there are decisions to be made that can greatly im
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Creation of a Small High-Throughput Screening Facility,d organizational effort. Major issues to consider include the selection of equipment, the establishment of data analysis methodologies, and the formation of a group having the necessary competencies. If done properly, it is possible to build a screening system in incremental steps, adding new pieces
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Enzyme Assay Design for High-Throughput Screening, initial active chemical compounds. The development of fluorescent- or absorbance-based readouts typically remains the formats of choice for enzyme screens and a wealth of experience from both industry and academia has led to a comprehensive set of standardized assay development and validation guide
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