Titlebook: Handbook of ELISPOT ; Methods and Protocol Alexander E. Kalyuzhny Book 2024Latest edition The Editor(s) (if applicable) and The Author(s),

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Pedro Telhado Pereira,Maria Eugénia Mataection and/or vaccination. Here, we describe in detail the methodology for using this ELISPOT assay to quantify SARS-CoV-2 spike-specific memory B cells produced by infection and/or vaccination in human PBMC samples. Application of this assay may help better understand and predict SARS-CoV-2 recall

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https://doi.org/10.1057/9780230119581x vivo, revealing its immunoglobulin class/subclass utilization, and its affinity distribution for the original, and for variant viruses/antigens. Because such comprehensive B?cell ImmunoSpot. tests can be performed with minimal cell material, are scalable, and robust, they promise to be well-suited

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