Titlebook: Handbook of ELISPOT ; Methods and Protocol Alexander E. Kalyuzhny Book 2024Latest edition The Editor(s) (if applicable) and The Author(s),

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Danilo Palazzo,Frederick Steinerunctions. Production of the appropriate Ig class/subclass is critical for both successful host defense and avoidance of immunopathology. The assessment of an antigen-specific B cell response, including its magnitude and Ig class/subclass composition, is most often confined to the antibodies present

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https://doi.org/10.1007/978-3-642-88583-9ngle-cell level. However, traditional ELISPOT based on enzyme-substrate color development can only detect one target. Therefore, scientists developed multiple-target ELISPOT based on enzyme-substrate coloring. Besides, FluoroSPOT that can detect 2–4 fluorescent signals are developed. Nevertheless, t

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Important Considerations for ELISpot Validation,ls in a straightforward and easy-to-learn approach. Its use in basic research, translational, and clinical work has been documented in countless publications. Harmonization guidelines and invaluable tools for optimal assay performance and evaluation exist. However, the validation of an established E

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An Overview of Peptides and Peptide Pools for Antigen-Specific Stimulation in T-Cell Assays,ISPOT), which depend on antigen-specific stimulation, increasingly use peptides to represent the antigen of interest. Besides single peptides, mixtures of peptides (peptide pools) are very frequently applied. Such peptide pools may, for example, represent entire proteins (with overlapping peptides c

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The Applications of ELISpot in the Identification and Treatment of Various Forms of Tuberculosis anidely used for the diagnosis of various infectious diseases, e.g., tuberculosis and sarcoidosis, and it is also widely used in cancer immunotherapy research. Its ability to distinguish between active and latent forms of tuberculosis makes it an extremely powerful tool for epidemiological studies and

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