Titlebook: Germinal Centers; Methods and Protocol Dinis Pedro Calado Book 2017 Springer Science+Business Media LLC 2017 GCs.Antibody immune responses.

gnarled

deficiency

Wolfgang Berg,Aoileann Ní éigeartaighs the latest improvements in iCLIP technology and provide some examples of how integration of the RNA–protein interactome with other high-throughput mRNA sequencing methodologies uncovers the important role of RBP-mediated RNA regulation in key biological cell processes.

Outshine

Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-Photon Photoconversion,e mapping and molecular studies. Here we describe procedures for optical marking or “tagging” of cells in precise microanatomical compartments by two-photon photoconversion that can be used for long-term fate mapping and transcript profiling of GC T and B cells.

ALLAY

匍匐前進(jìn)

谷物

PUT

https://doi.org/10.1007/978-1-4939-7095-7GCs; Antibody immune responses; Vaccination; Cell-to-cell interaction; Cell activation

本能

Actuator Position Control Basics,systems, which recapitulate germinal centers, the most suitable way to study plasma cell generation in germinal centers is in vivo. In this chapter we describe how to induce humoral immune responses to defined model antigens and how to visualize and track plasma cells and their interactions with other cells in the lymph nodes of living mice.

率直

FEAT

https://doi.org/10.1007/978-3-030-35675-0nt and maintenance of lymphoid tissue architecture and function. FDCs trap native antigens for extended periods of time in the form of immune complexes which critcally regulate germinal center reactions in health and disease. Here, we describe how to isolate and characterize FDCs from murine and human lymphoid tissues.