Titlebook: Germ Cell Development; Methods and Protocol Marco Barchi,Massimo De Felici Book 2024 The Editor(s) (if applicable) and The Author(s), under

BOON

Axillary

Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cprinciple that gene knockout can be effectively targeted in mouse germline stem cells by lipofecting Cas9:gRNA ribonucleoproteins. In this protocol, we describe a simple, fast, and cheap workflow for gene editing via the lipofection of non-integrative ribonucleoproteins in murine male germline stem cells.

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Histological and Cytological Techniques to Study Perinatal Mouse Ovaries and Oocytesing ovaries and oocytes play an essential role in the study of mammalian follicle development and oocyte quality. This chapter presents a collection of protocols that focus on various methods for studying mammalian ovaries and oocytes, providing researchers with a variety of approaches to choose from.

Instantaneous

Isolation and Purification of Viable PGCs from Mouse Embryosc information to subsequent generations. Studies aimed to unravel PGC development at molecular level in mammals can be traced at the early 1980s and were hampered by the difficulty in obtaining both sufficient quantities and purity of PGCs. For many laboratories, the isolation and purification metho