Titlebook: Germ Cell Development; Methods and Protocol Marco Barchi,Massimo De Felici Book 2024 The Editor(s) (if applicable) and The Author(s), under

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Method of Isolation and In Vitro Culture of Primordial Follicles in Bovine Animal Model homogeneous population of viable primordial follicles suitable for in vitro culture. Here, we provide a fast and high-yield strategy for the mechanical isolation of primordial follicles from limited portions of the ovarian cortex in the bovine animal model.

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北極熊

1064-3745 expertsThis detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and i

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Emp?rung, Wahrhaftigkeit und Common Sensedevelopmental stages. Here, we describe in detail a method based on the Percoll gradient, routinely used in our laboratory, allowing us to obtain from blood and gonad anlages significant numbers of viable PGCs which can be successfully cultured or efficiently genetically modified.

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Eugène Dubois and the Ape-Man from Java model. We describe a stepwise protocol to induce epiblast-like cells and subsequent PGCLCs by forming spherical aggregates from rat PSCs. We also describe a protocol to mature these PGCLCs from specified/migratory to the gonadal stage by aggregation with female gonadal somatic cells.