Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2017 Springer Science+Business Media LLC 2017 enzymes.TALEN.ZFN.CRISPR.ze

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Vincent C. S. Wiers,A. (Ton) G. de Kokes into embryos using conventional microinjection and a new electroporation method named Technique for Animal Knockout system by Electroporation (TAKE). This chapter introduces the latest protocols for producing genetically engineered rats using genome editing.

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Genome Editing in Mouse and Rat by Electroporation,and high-efficiency methods that do not require special skills such as microinjection is needed. This chapter presents a new technique called Technique for Animal Knockout system by Electroporation (TAKE), which produces genome-edited rodents by direct introduction of engineered endonucleases into intact embryos using electroporation.

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Genome Editing of Rat,es into embryos using conventional microinjection and a new electroporation method named Technique for Animal Knockout system by Electroporation (TAKE). This chapter introduces the latest protocols for producing genetically engineered rats using genome editing.

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古文字學(xué)

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1064-3745 ce from the experts.Includes supplementary material: .Includ.This volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Writte

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Robert Freestone,Gethin Davison,Richard Hu a wide range of deployments for clinical and industrial applications. This article introduces a ZFN construction protocol that uses bacterial one-hybrid (B1H) selection and single-stranded annealing (SSA) assay.