Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2017 Springer Science+Business Media LLC 2017 enzymes.TALEN.ZFN.CRISPR.ze

光明正大

Designing with Structural CeramicsCRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.

FILTH

確定方向

CRISPR/Cas9,CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.

monochromatic

Genome Editing of Mouse by Cytoplasmic Injection,CRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.

玩忽職守

Genome Editing of Medaka,Medaka (.), along with zebrafish (.), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.

進(jìn)入

Melanoma

Power Analysis and Optimization,we recently established the system for genome editing in embryonic stem (ES) cells and its application for chimeric analysis in mice. In this section, we introduce the procedure for genome editing in mouse zygotes and ES cells.

拍翅

lambaste

Radiation