Titlebook: Genetically Incorporated Non-Canonical Amino Acids; Methods and Protocol Yu-Hsuan Tsai,Simon J. Els?sser Book 2023 The Editor(s) (if applic

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1064-3745 expertsThis detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in .E. coli., mammalian cells, and animals, highlighting specific applications r

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Monitoring Your Containerized Environment,account of the incorporation of sTyr in HEK293T cells using the enhanced green fluorescent protein as an example. This method can be widely applied to incorporating sTyr into any POI to investigate the biological functions of PTS in mammalian cells.

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Repurposing Photosensitizer Proteins Through Genetic Code Expansion to Facilitate Photo-Biocatalysispurification, and characterization of the . are detailed. The installation of the catalytic modules and the utilization of .-based artificial photoenzymes for photoenzymatic CO. reduction and dehalogenation are also described.

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Book 2023d Non-Canonical Amino Acids: Methods and Protocols. serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering..