Titlebook: Genetically Incorporated Non-Canonical Amino Acids; Methods and Protocol Yu-Hsuan Tsai,Simon J. Els?sser Book 2023 The Editor(s) (if applic

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Encoding Noncanonical Amino Acids into Phage Displayed Proteinsalities and building blocks in proteins displayed on phage provides the foundation for further phage display applications in fields such as imaging, protein targeting, and the production of new materials.

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Conformational GPCR BRET Sensors Based on Bioorthogonal Labeling of Noncanonical Amino Acidsonal sensors based on the minimally invasive bioorthogonal labeling procedure, we describe a method that can be used in a microtiter plate format and can be easily adopted to investigate ligand-induced dynamics in various membrane receptors.

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Fault-Tolerant System Technology,eplacement with photocaged tyrosine (pcY). This is followed by cloning of plasmids and expression of pcY-containing antibody fragments in .. Finally, we describe a cost-effective and biologically-relevant method for measuring the binding affinity of photoactive antibody fragments to antigens expressed on the surface of live cancer cells.

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Nonrenewable Two-State Systems,minocoumarin-derived fluorescent ncAA into proteins in . and preparation of a fluorescent ncAA-based FRET probe for assaying the?activities of deubiquitinases, a key class of enzymes involved in ubiquitination. We also describe the deployment of an in vitro fluorescence assay to screen and analyze small-molecule inhibitors against deubiquitinases.

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