Titlebook: Gap Junction Protocols; Mathieu Vinken,Scott R. Johnstone Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 Con

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Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junto communicate between cells and organs, including the brain, lung, liver, lens, retina, and heart. Gap junctions enable a direct route for ions like calcium and potassium, and low molecular weight compounds, such as inositol 1,4,5-trisphosphate, cyclic adenosine monophosphate, and various kinds of

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Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gaplls with resolution to the single channel level. The dual patch clamp recording technique requires specialized equipment and the acquired skill to reliably establish gigaohm seals and the whole cell recording configuration with high efficiency. This chapter describes the equipment needed and methods

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https://doi.org/10.1007/978-1-4939-3664-9Connexin expression; Cell signaling; Connexons; Gap junctional intercellular communication; GJIC regulat

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https://doi.org/10.1007/978-1-4757-4523-8hylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in huma

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Optimization Algorithms and Applications,the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modification

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https://doi.org/10.1007/978-94-6209-332-4ferent connexin isoforms. Thus, specific connexin gene knockdown using RNAi-mediated technologies is a technique that allows investigators to efficiently monitor silencing effects of single or multiple connexin gene products. The present chapter describes the transient knockdown of connexins in vitr

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