Titlebook: Gap Junction Protocols; Mathieu Vinken,Scott R. Johnstone Book 2016 Springer Science+Business Media, LLC, part of Springer Nature 2016 Con

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Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Ceferent connexin isoforms. Thus, specific connexin gene knockdown using RNAi-mediated technologies is a technique that allows investigators to efficiently monitor silencing effects of single or multiple connexin gene products. The present chapter describes the transient knockdown of connexins in vitr

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Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins fo mechanisms, the use of cell culture systems is essential to discriminate between the effects of different connexin isoforms expressed in individual cells or tissues of the developing conceptus or in maternal reproductive tissues. The generation and analysis of gene-deficient trophoblast stem cell l

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Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Couplingor the maintenance of tissue homeostasis. Gap junction channels are protein channels that are situated between neighboring cells and that provide a direct, yet selective route for the passage of small hydrophilic biomolecules and ions. Here, an electroporation method is described to load a localized

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Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro,gh initially developed to investigate intracellular mobility, FRAP can be also used to measure intercellular dynamics. This chapter describes how to perform FRAP experiment to study gap junctional communication in living cells. The procedures described here can be carried out with a laser-scanning c

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