Titlebook: Galectins; Methods and Protocol Sean R. Stowell,Richard D. Cummings Book 2015 Springer Science+Business Media New York 2015 galectin funct

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Sean R. Stowell,Richard D. CummingsIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia

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Evolving Mechanistic Insights into Galectin Functions,ears ago, ideas about their biological functions continue to evolve. Current evidence indicates that galectins, which are the only known GBPs that occur free in the cytoplasm and extracellularly, are involved in a variety of intracellular and extracellular pathways contributing to homeostasis, cellu

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Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporion. While a variety of approaches can be utilized to prevent galectin oxidation, several of these require inclusion of reducing agents that not only prevent galectins from undergoing oxidative inactivation, but can also interfere with normal redox potentials required for fundamental cellular proces

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,Probing Lectin–Mucin Interactions by Isothermal Titration Microcalorimetry,stant, binding stoichiometry, and heat of binding (enthalpy) and indirectly the entropy and free energy of binding. ITC has been extensively used to study the binding of lectins to mono- and oligosaccharides, but limited applications to lectin–glycoprotein interactions. Inherent experimental challen

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