Titlebook: Galectins; Methods and Protocol Sean R. Stowell,Richard D. Cummings Book 2015 Springer Science+Business Media New York 2015 galectin funct

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Moral Lessons in Aeschylean Drama to target specifically the complex formation during GAL1-induced pre-BCR clustering and lattice formation, using immunofluorescence techniques. Moreover, an in vivo assay was set up to study the consequence of synapse formation on the early steps of B cell development.

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Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis, the potential impact of alterations in glycosylation on carbohydrate recognition. Using these approaches, we present examples of flow cytometric and fluorescence-based solid phase assays to study galectin–carbohydrate interactions.

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Examination of the Role of Galectins in Plasma Cell Differentiation,. fusion proteins for determining their binding to B cells, introduction of ectopic galectins in primary B cells using retroviral vectors, and inhibition of the binding of galectins to B cells by synthetic disaccharides.

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Fourier optics of aerosol media,ver prolonged periods of time and retains the carbohydrate binding and biological activities of the native protein. As a result, this approach allows examination of the biological roles of a stabilized form of galectin-1 without introducing the confounding variables that can occur when typical soluble reducing agents are employed.

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