Titlebook: Extracellular Vesicles in Diagnosis and Therapy; Maurizio Federico,Barbara Ridolfi Book 2022 The Editor(s) (if applicable) and The Author(

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Purification and Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesiclesethod for protein extraction, and PolyMAC-based enrichment of phosphopeptides. The combination of these methods provides a highly effective strategy for EV-based phosphoproteome analysis and leads to the discovery of novel phospho-markers previously undetectable.

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Maurizio Federico,Barbara RidolfiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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https://doi.org/10.1007/978-1-0716-2341-1Disease biomarkers; EV detection and isolation; Macromolecule delivery; Biofluids; Vesicle engineering; I

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Extracellular Vesicles in Diagnosis and Therapy978-1-0716-2341-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Victor J. Ferrans,Jean-Fran?ois Bernaudinxosomes are potential biomarkers for cancer diagnosis. Biosensors are useful analytical tools for quantification of biomarkers and targeted molecules. An aptasensor uses the aptamer as the biorecognition element to bind to the target and is one main type of biosensors that is promising for exosomes

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https://doi.org/10.1007/978-3-030-20327-6ifferent cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a s