Titlebook: Extracellular Vesicles in Diagnosis and Therapy; Maurizio Federico,Barbara Ridolfi Book 2022 The Editor(s) (if applicable) and The Author(

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Isolation and Characterization of Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Dely, an immunoaffinity-based EV separation method followed by EV characterization, quantification, and normalization, as well as consecutive miRNA isolation and miRNA profiling by small RNA sequencing, are described.

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Extracellular Vesicles and Their Use as Vehicles of Immunogensting enveloped viral vectors and virus-like particles. Finally, we describe a both safe and original approach conceived for the induction of strong CTL immunity against antigens uploaded in EVs constitutively released by muscle cells.

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1064-3745 agnosis and Therapy. serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication..978-1-0716-2343-5978-1-0716-2341-1Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-319-11155-1 the mouse melanoma cell line B16F10 that yields highly enriched EV samples for subsequent applications such as molecular and functional studies. Our protocol also includes an optional labeling step with the lipophilic dye DiD, which allows tracking of EV uptake by recipient cells in vitro and in vivo.

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Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured Tumor Cells the mouse melanoma cell line B16F10 that yields highly enriched EV samples for subsequent applications such as molecular and functional studies. Our protocol also includes an optional labeling step with the lipophilic dye DiD, which allows tracking of EV uptake by recipient cells in vitro and in vivo.

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Indurate