Titlebook: Exocytosis Methods; Peter Thorn Book 2014 Springer Science+Business Media New York 2014 Exocytosis control.Hormone release.Imaging.Microsc

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Carbon-Fiber Amperometry in the Study of Exocytosis many significant biological pathways. These molecules, including neurotransmitters, hormones, growth factors, and peptides, are released from cells via a process called exocytosis. Our laboratory has utilized a noninvasive method of measuring the release of oxidizable molecules from cells, known as

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Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells which reflect membrane area changes due to fusion or fission of secretory vesicles. This parameter can be monitored directly by the electrophysiologic patch-clamp technique developed by Erwin Neher and Bert Sakmann in 1976. It is still widely used, primarily for the study of currents across ion cha

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Measuring Exocytosis in Endocrine Tissue Slicesdescription of a selection of the methods that have been adapted to study regulated exocytosis in endocrine tissue slices is provided in this chapter. Such adaptation of the methods has been associated with a plethora of open issues that required innovative solutions, but innovation has been and sha

mucous-membrane

Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Li recent application of intravital microscopy to image subcellular structures in vivo has enabled researchers to investigate the machinery controlling regulated exocytosis in live rodents. Here, we describe selected experimental models that have been used to investigate the dynamics of the secretory