Titlebook: Exocytosis Methods; Peter Thorn Book 2014 Springer Science+Business Media New York 2014 Exocytosis control.Hormone release.Imaging.Microsc

陶醉

clarify

https://doi.org/10.1007/978-3-662-07346-9 many significant biological pathways. These molecules, including neurotransmitters, hormones, growth factors, and peptides, are released from cells via a process called exocytosis. Our laboratory has utilized a noninvasive method of measuring the release of oxidizable molecules from cells, known as

SHOCK

https://doi.org/10.1007/978-3-662-68025-4s at the level of exocytosis of insulin, transcriptional rate of the insulin gene, translation of the mRNA, and processing of the proinsulin to mature insulin (Mahato et al., Biochem J 174:517–526, 1978; Hedeskov, Physiol Rev 60:442–509, 1980; Welsh et al., J Biol Chem 260:13590–13594, 1985; Welsh e

AVANT

天真

https://doi.org/10.1007/978-3-662-25758-6 which reflect membrane area changes due to fusion or fission of secretory vesicles. This parameter can be monitored directly by the electrophysiologic patch-clamp technique developed by Erwin Neher and Bert Sakmann in 1976. It is still widely used, primarily for the study of currents across ion cha

搬運工

Funktionelle Pathologie in der Chirurgiedescription of a selection of the methods that have been adapted to study regulated exocytosis in endocrine tissue slices is provided in this chapter. Such adaptation of the methods has been associated with a plethora of open issues that required innovative solutions, but innovation has been and sha

Epithelium

,Zyklische Ver?nderungen am Uterus, recent application of intravital microscopy to image subcellular structures in vivo has enabled researchers to investigate the machinery controlling regulated exocytosis in live rodents. Here, we describe selected experimental models that have been used to investigate the dynamics of the secretory

Aesthete

Neuromuscular Dysfunction and Paraplegia,lecules that target SVE may prove to be useful not only for basic research into synaptic function but also in treating diseases that involve pathological neurotransmission. However, there are relatively few small molecules targeting SVE that have known molecular targets, and the development of these

dandruff

https://doi.org/10.1007/3-211-30826-1ituitaries). These nerve terminals release the two neuropeptides, oxytocin and vasopressin, which are easily assayed by radioimmunoassay and/or ELISA. Depolarization-induced exocytosis is dependent on the same parameters as those regulating release from the whole, intact neurohypophysis. The isolate

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