Titlebook: Enhancers and Promoters; Methods and Protocol Tilman Borggrefe,Benedetto Daniele Giaimo Book 2021 The Editor(s) (if applicable) and The Aut

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Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes population of heterotypic H2A.Z–H2A containing nucleosomes. This protocol can be adopted to investigate any pair-wise combination of any histone variant, histone posttranslational modification, or any other protein that binds to a modified nucleosome.

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Analysis of Enhancer–Promoter Interactions using CAGE and RADICL-Seq TechnologiesA interacting complexes ligated and sequenced (RADICL-Seq) a novel methodology to map genome-wide RNA–chromatin interactions in intact nuclei. Here, we describe how CAGE and RADICL-Seq data can be used to characterize enhancer elements and identify their target genes.

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978-1-0716-1599-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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The Physical Basis of Biochemistryntrol, methods are required that track genome transcription with high precision in vivo. Here, we provide step-by-step guidance for performing native elongating transcript sequencing (NET-Seq) in mammalian cells. NET-Seq allows quantitative measurements of transcription genome-wide, including enhanc

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