Titlebook: Enhancers and Promoters; Methods and Protocol Tilman Borggrefe,Benedetto Daniele Giaimo Book 2021 The Editor(s) (if applicable) and The Aut

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書目名稱Enhancers and Promoters影響因子(影響力)




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Global Run-on Sequencing (GRO-Seq) RNA, such as mRNAs, rarely reflect accurately the rate of in situ transcription in nuclei by RNA polymerases (RNAPs). The “Global Run-on Sequencing (GRO-Seq)” method, developed in 2008, combines the nuclear run-on assay with next-generation deep sequencing to detect nascent RNA levels to annotate t

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Motilin

Hyaluronic-Acid

Hyaluronic-Acid

inspired

Low Input Targeted Chromatin Capture (Low-T2C)ed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinf

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Seminar

Analysis of Enhancer–Promoter Interactions using CAGE and RADICL-Seq Technologiesrch. Enhancer–promoter interactions are believed to mediate activation of target genes. Bidirectional transcription represents one hallmark of active enhancers that can be measured using transcriptome technologies such as Cap analysis of gene expression (CAGE). Recently, we have developed RNA and DN