Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson ?rom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge

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1064-3745 ation advice from the experts.This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and explo

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Book 2017and tips on troubleshooting and avoiding known pitfalls..?. .Authoritative and cutting-edge, .Enhancer RNAs: Methods and Protocols. aims to ensure successful results in this rapidly developing field..

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https://doi.org/10.1057/9780230306479ased Peak Identification (DPI) and the PROmiRNA software. We apply both methodologies to the challenging task of identifying primary microRNAs transcript (pri-miRNA) start sites and compare the results.

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Identification of Transcribed Enhancers by Genome-Wide Chromatin Immunoprecipitation Sequencing, cloud downstream analysis. Here we present a protocol that combines wet and dry bench methods to accurately identify transcribed enhancers genome-wide as well as an experimental procedure to validate these datasets.

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Computational Approaches for Mining GRO-Seq Data to Identify and Characterize Active Enhancers,re-processing, alignment, and transcript calling. In addition, we describe protocols and computational pipelines for mining GRO-seq data to identify active enhancers, as well as known transcription factor binding sites that are transcribed. Furthermore, we discuss approaches for integrating GRO-seq-

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Evaluating the Stability of mRNAs and Noncoding RNAs,ion of yet uncharacterized regulatory ncRNAs. In this chapter, we discuss the methodologies currently used to estimate RNA decay and outline an experimental protocol for genome-wide estimation of RNA stability of protein-coding and lncRNAs. This protocol details the transcriptional blockage of cultu