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Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson ?rom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge

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發(fā)表于 2025-3-21 18:13:40 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Enhancer RNAs
副標(biāo)題Methods and Protocol
編輯Ulf Andersson ?rom
視頻videohttp://file.papertrans.cn/312/311270/311270.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Enhancer RNAs; Methods and Protocol Ulf Andersson ?rom Book 2017 Springer Science+Business Media New York 2017 long ncRNAs.transcription.ge
描述.This volume provides a comprehensive overview of the experimental and computational methodologies used to study the function of long non-coding RNA (ncRNAs) expressed from enhancers. Chapter detail both wet-lab and dry-lab techniques and annotating long ncRNAs and exploring transcription by assessing where transcription starts and generally how it occurs.Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..?. .Authoritative and cutting-edge, .Enhancer RNAs: Methods and Protocols. aims to ensure successful results in this rapidly developing field..
出版日期Book 2017
關(guān)鍵詞long ncRNAs; transcription; genome-wide; RNA manipulation; siRNA; antisense oligos
版次1
doihttps://doi.org/10.1007/978-1-4939-4035-6
isbn_softcover978-1-4939-8160-1
isbn_ebook978-1-4939-4035-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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發(fā)表于 2025-3-21 21:31:43 | 只看該作者
Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/311270.jpg
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The philosophy of Jules Lachelierr-derived long noncoding RNAs (long ncRNAs), including enhancer RNAs (eRNAs), are an important component of enhancer function, their expression has not been broadly analyzed at a single cell level via imaging techniques. This protocol describes a method to image eRNA in single cells by in situ hybri
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https://doi.org/10.1007/978-1-349-19731-6 This protocol uses a small number of 22–28 nucleotide biotinylated antisense oligonucleotides, complementary to regions of the target RNA that are accessible for hybridization, to purify RNAs from a cross-linked chromatin extract. RNA–chromatin complexes are next immobilized on beads, washed, and s
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Carlos Moruj?o,Samuel Dimas,Susana Relvasction of noncoding RNAs at enhancers, termed eRNAs, correlates with higher expression of genes that the enhancer interacts with; hence, eRNAs provide a new tool to model gene activity in normal and disease tissues. Moreover, this unique class of noncoding RNA has diverse roles in transcriptional reg
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