Titlebook: Endocannabinoid Signaling; Methods and Protocol Mauro Maccarrone Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), u

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Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells,an bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB. receptor.

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,Assay of GTPγS Binding in Autoradiography,Autoradiography of radiolabeled GTPγS ([.S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) .. Here, we describe the protocol for such a method, suitable for investigating CB. receptor functionality in tissue slices from rodent brains.

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1064-3745 ing: Methods and Protocols, Second Edition .is a valuable resource for all researchers interested in learning more about this important and developing field..978-1-0716-2730-3978-1-0716-2728-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Reliability Analysis in the Design Phase,docannabinoids. The chapter contains a step-by-step protocol for the analysis of (n-3) endocannabinoids in plasma, including sample collection and solid phase extraction, LC–MS analysis, and data processing. In addition, protocol modifications are provided to allow quantification of n-3 PUFA-derived

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Complex System Reliability Analysis Methods,physiological effects at both central and peripheral levels, which may have therapeutic value in different human pathologies, then providing a solid experimental explanation for both the ancient medicinal uses of Cannabis plant and the recent advances in the development of cannabinoid-based specific

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