Titlebook: Embryo Models In Vitro; Methods and Protocol Magdalena Zernicka-Goetz,Kursad Turksen Book 2024 The Editor(s) (if applicable) and The Author

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Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome is chapter, we focus on the analysis of human blastoids, which model the blastocyst, and their integrative analysis with human embryo datasets and a 2D in vitro early development model system dataset, which models epiblast, extraembryonic mesoderm, and trophoblast cells.

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Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro Models, and maternal environment. Here, we present a protocol to obtain different types of placental trophoblast cells and trophoblast organoids using hTSCs. The generation of hTSC-organoids takes 6 days. hTSC-organoids permit passaging and can differentiate into EVT lineage.

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Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos,ucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.

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https://doi.org/10.1007/978-3-8350-9611-0k, a 3D organoid system called “somitoid” supporting the simultaneous formation of somite-like structures, and another organoid system called “segmentoid” reconstituting in vivo–like hallmarks of somitogenesis. Together, these complementary model systems provide an excellent platform to decode somitogenesis and advance human developmental biology.

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Detlef Angermann,Roland Pail,Urs Hugentoblerflush and smear to confirm successful mating, performing euthanasia of the guinea pig, and flushing in vivo embryos. Once collected, the embryos can be utilized for numerous downstream applications. Here we will cover embryo culturing and processing embryos for immunofluorescence.