Titlebook: Embryo Models In Vitro; Methods and Protocol Magdalena Zernicka-Goetz,Kursad Turksen Book 2024 The Editor(s) (if applicable) and The Author

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Generation of Stem Cell-Based Mouse Embryo-Like Structures,” ETiX-embryoids are formed from combined embryonic stem cells, trophoblast stem cells, and embryonic stem cells transiently induced to express Gata4. Cells are seeded into AggreWell dishes where they form aggregates that develop to resemble post-implantation mouse embryos following 4?days of cultur

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Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos,logical systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to el

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Evaluation of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell Transcriptome to capture biological heterogeneity and assess the cellular composition in early embryos. Comparative analysis of the transcriptional landscapes of embryos with single-cell resolution allows us to better understand and improve stem-cell-based embryo models. However, proper comparison between differ

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Inferring Gene Regulatory Networks and Predicting the Effect of Gene Perturbations via IQCELL, and perturbed conditions. In this chapter, we provide a detailed guideline for implementing IQCELL from a raw dataset. The steps include processing data, inferring informative genes, inferring gene regulatory network, and simulating the resulted network under normal and perturbed conditions.

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Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence,nslate findings to human development, it is crucial to select an appropriate animal model that closely resembles the specific aspect of development under study. The guinea pig is highlighted as a useful platform for reproductive studies due to similarities in in utero development and general physiol

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