Titlebook: Embryo Culture; Methods and Protocol Gary D. Smith,Jason E. Swain,Thomas B. Pool Book 2012 Springer Science+Business Media, LLC 2012 cell f

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Utility of Animal Models for Human Embryo Culture Development: Domestic Species, of many domestic species as more relevant, applicable models of early human development. Here, we describe the advantages and disadvantages of domestic species as animal models to develop in vitro culture methods and conditions that successfully support human embryo development. In vitro embryo cul

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Utility of Animal Models for Human Embryo Culture: Nonhuman Primates,dying rodents and other systems, it is impossible to envision how those mechanistic findings can be responsibly translated to the clinic without the appropriate use of nonhuman primates. Thankfully, noninvasive technologies now permit nonhuman primate studies without endangering the model itself. Wo

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Media Composition: Amino Acids and Cellular Homeostasis,tically review the importance of amino acids for preimplantation embryos and the impact of this research for the development of sequential culture media used in many assisted conception units. The advantages of culturing embryos in a full complement of amino acids, at close to physiological concentr

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Media Composition: Macromolecules and Embryo Growth,ably the most common supplement followed by globulin-enriched preparations. Serum supplementation and Co-Culture of embryos belong to the past. Defined nonprotein or recombinant protein supplements are becoming a viable alternative during gamete and embryo manipulation procedures. Biological protein

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Media Composition: Antioxidants/Chelators and Cellular Function,pecies (ROS) is triggered by various components of the in vitro environment, most of which embryos do not normally encounter in vivo. To compensate for these deficiencies in the culture environment, antioxidants and chelators are often used to control or suppress ROS levels as embryos develop. Howev

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